The Single-Tube Magnet is a convenient separation device for cell separation, magnetocapture, or immunoassays using BioMag magnetic particles in a 1.5 ml or 2 ml tube. The beads are pulled to the side of the tube by the magnetic field, which has a strength of greater than 20 megaoersted, and held in place while solutions are exchanged or removed. The Single-Tube Magnet can separate volumes between 40 µl and 1 ml that contain <5 mg BioMag particles. Removal of the magnetic field allows easy resuspension of the magnetic particles.