QIAamp DSP Virus Kit

For purification of viral nucleic acids from human plasma and serum for in vitro diagnostic use
  • Universal purification system compatible with other IVD products
  • Hiqh-quality viral nucleic acids
  • Concentration with 20 µl or 60 µl eluates
  • Sample volumes of 500 µl
  • Rapid purification and minimal risk of cross-contamination

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.

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QIAamp DSP Virus Kit
For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors
 60704
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The QIAamp DSP Virus Kit is intended for in vitro diagnostic use.


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QIAamp DSP Virus Kit procedure.|Universal nucleic acid purification system that easily fits into diagnostic workflows.|
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Performance

Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification or other modification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure
The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute.  Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, "Procedure").
Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.

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Feature
Specifications
Applications PCR, RT-PCR, LCR
CE/FDA/IVD compatible CE/IVD
Elution volume 20 µl, 60 µl
Format Sample tubes
Main sample type Serum, plasma
Processing Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 500 µl
Technology Silica technology
Time per run or per prep 25 minutes
Yield Varies

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