QIAamp RNA Blood Mini Kit

For purification of cellular RNA from fresh whole blood
  • Rapid purification of high-quality, ready-to-use RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. Purification can be fully automated on the QIAcube.

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QIAamp RNA Blood Mini Kit (50)
For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free reagents and buffers
 52304
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The QIAamp RNA Blood Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp RNA Blood Mini procedure.|High-quality RNA for northern analysis.|Reliable RT-PCR analysis.|
|Formaldehyde agarose gel and corresponding northern blot (GAPDH-probed) of total RNA isolated with the QIAamp RNA Blood Mini Kit from 0.5, 1.0, and 1.5 ml (left to right) of healthy whole human blood (with indicated anticoagulants). Forty microliters of a 60 µl eluate were loaded per lane. M: 0.24–9.5 kb RNA ladder.|RT-PCR of total RNA from blood. Total RNA from 50 µl of healthy whole human blood (with indicated anticoagulants) was purified using the QIAamp RNA Blood Mini Kit and eluted with 50 µl of RNase-free water. For each sample, three RT-PCR reactions were performed (left to right) using 5 µl or 15 µl of the eluate or a control (15 µl eluate, RT omitted). For RT-PCR, samples were digested with RNase-free DNase and reverse transcription performed using an oligo-dT primer. 1/10 (2.5 µl) of the cDNA was amplified using GAPDH primers. 1/5 of the PCR reaction was loaded per lane. – PCR was performed as above but eluate was replaced with distilled water; + positive control cDNA fragment. M: 100 bp DNA ladder.|
Performance

The QIAamp procedure completely removes RNases, contaminants, and enzyme inhibitors, yielding high-quality RNA suitable for any downstream application (see figures "High-quality RNA for northern analysis" and "Reliable RT-PCR analysis").

The QIAamp RNA Blood Mini Kit provides the highest-quality RNA with minimum copurification of DNA. However, as with any RNA purification method, some DNA contamination can be expected. For certain RNA applications that are sensitive to very small amounts of DNA, it may be necessary to remove any remaining DNA. In these cases, the QIAGEN RNase-Free DNase Set provides convenient on-column DNase treatment of RNA samples during QIAamp RNA procedures.

Principle

The QIAamp RNA Blood Mini Kit provides purification of cellular RNA using silica-membrane technology. No phenol–chloroform extraction is required. RNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure RNA to be eluted in either water or a buffer provided with the kit.

QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

Procedure
The QIAamp RNA Blood Mini Kit simplifies isolation of RNA from blood with a fast spin-column procedure (see figure "Procedure"). Red blood cells are selectively lysed and white cells collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the QIAshredder spin column, the sample is applied to the QIAamp spin column. Total RNA binds to the QIAamp membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100 µl RNase-free water (provided with the kit) for direct use in any downstream application.
Applications

The QIAamp RNA Blood Mini Kit is designed for isolation of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. In addition, total cellular RNA can be isolated from tissue samples.

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Feature
Specifications
Applications PCR, real-time PCR, microarray
Elution volume 30–100 µl
Format Spin columns
Main sample type Whole blood, tissue
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Cellular RNA
Sample amount 50–1.5 ml
Technology Silica technology
Time per run or per prep <1 hour
Yield 1–5 µg

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Kit Handbooks
1
QIAamp RNA Blood Mini Handbook - (EN)
For total RNA purification from human whole blood
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