AllPrep DNA/RNA 96 Kit

By allowing the analysis of DNA and RNA from the same sample, the AllPrep DNA/RNA 96 Kit is the ideal tool for sample preparation in genomics and systems biology. Efficient purification of high-quality DNA and RNA from different tissue types is achieved without the need for additional RNase or DNase digestion. Both cultured cells and easy-to-lyse tissues can be processed. The kits form part of QIAGEN's solution for preparing multiple analytes from the same sample.

A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart "AllPrep DNA/RNA procedure"). The 96-well purification plates of the kit are for DNA and RNA (see figure "AllPrep RNA 96-well plate"; DNA plate not shown). The plates are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-16 and Plate Rotor 2 x 96) or a combination of vacuum (QIAvac 96) and centrifuge. Both cultured cells and easy-to-lyse tissues can be processed.

The kits form part of QIAGEN's solution for preparing multiple analytes from the same sample. These include Allprotect Tissue Reagent, which stabilizes DNA, RNA, and protein in tissues, and RNAprotect Cell Reagent, which stabilizes DNA and RNA in cells. Both reagents deliver immediate stabilization at room temperature. For tissues, TissueRuptor and TissueLyser systems provide fast disruption at low- to high-throughputs.

The AllPrep DNA/RNA 96 Kit is the ideal tool for sample preparation in genomics and systems biology. The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is well suited for any application, including Southern-, dot-, and, slot-blot analyses; DNA methylation analysis; and PCR and multiplex PCR. The purified total RNA is ready to use in any application, such as RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarray analysis.