QIAsymphony DNA Kits

Total DNA was purified from human whole blood (3 donors, 6 replicates each) on the QIAsymphony SP using the QIAsymphony DNA Mini Kit (200 µl) or QIAsymphony DNA Midi Kit (1000 µl). Blood was collected in BD Vacutainer K2E tubes and stored at 5–8°C prior to use. Yield was determined by spectrophotometric analysis.|A 1 μl aliquot of eluate was used for amplification of a 1.2 kb fragment of the hugl gene. PCR was performed using QIAGEN HotStarTaq DNA Polymerase. No PCR inhibition was detected with 1 μl eluate per reaction. M: markers.|A 10 μl aliquot of eluate was used for amplification of a 1.2 kb fragment of the hugl gene. PCR was performed using QIAGEN HotStarTaq DNA Polymerase. No PCR inhibition was detected with 10 μl eluate per reaction. M: markers.|Spectrophotometric analysis demonstrated the high purity of DNA isolated on the QIAsymphony SP.|The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
|Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use giving you complete cost control. 
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