Investigator ESSplex SE GO! Kit

For multiplex amplification of the new European Standard Set of loci, plus SE33, and Amelogenin directly from swabs and papers
  • Highly suitable for reference samples from buccal cells/blood on FTA paper or buccal swabs
  • Low price per result in a high throughput reference setup
  • Fastest direct amplification workflow in forensics and paternity analysis
  • Straightforward protocols with ready-to-use Fast Reaction Mix
  • Simultaneous amplification of 17 loci

The Investigator ESSplex SE GO! Kit amplifies the full suite of 15 STR markers in the new European Standard Set (ESS) of loci, plus SE33, and the gender-specific Amelogenin, as recommended by the European Network of Forensic Science Institutes (ENFSI) and approved by the European Union Council. The kit utilizes a modified version of QIAGEN’s fast-cycling plus technology, enabling amplification in as little as 45 minutes. With rapid and reliable results from reference samples such as buccal swabs, as well as buccal cells or blood on papers, the Investigator ESSplex SE GO! Kit is suitable for reference testing in forensic applications and paternity testing.
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Product Cat. no. List price:
Investigator ESSplex SE GO! Kit (200)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE GO!, DNA size standard 550 (BTO)
 381566
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Investigator ESSplex SE GO! Kit (1000)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE GO!, DNA size standard 550 (BTO)
381568
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Investigator STR GO! Lysis Buffer (200)
Lysis buffer for 200 swab samples
 386516
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Investigator STR GO! Punch Buffer (1000)
Lysis buffer for 1000 samples of epithelial cells on paper
 386528
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Investigator STR GO! Punch Buffer (200)
Lysis buffer for 200 samples of epithelial cells on paper
 386526
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The Investigator ESSplex SE GO! Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease. This product is not available in all countries.

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Performance
The Investigator ESSplex SE GO! Kit utilizes fast-cycling technology to provide reliable results in approximately 45 minutes, which is significantly faster than kits from other suppliers.

The novel Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been further optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium, and collagen) shows high sensitivity and superior inhibitor resistance. This enables the generation of reliable, high-quality results for example directly from blood or for samples on FTA paper.

The Investigator ESSplex SE GO! Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
Principle
The Investigator ESSplex SE GO! Kit uses QIAGEN's fast-cycling PCR technology for the direct, simultaneous amplification of the 15 STR markers in the new European Standard Set (ESS) of loci, plus SE33, and the gender-specific Amelogenin. Carefully designed primers prevent overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.
Procedure
The 16 polymorphic STR markers and the gender-specific Amelogenin are amplified simultaneously in a single PCR directly from swabs or papers and labeled with one of the dyes listed (see the table Investigator ESSplex SE GO! Kit dyes and corresponding markers).

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software (see the table Technical specifications for more information). 

Investigator ESSplex GO! Kit dyes and corresponding markers

Dye

Marker

 

 

 

 

6-FAM

Amelogenin

TH01

D3S1358

vWA

D21S11

BTG

D16S539

D1S1656

D19S433

SE33

 
BTY D10S1248  D22S1045  D12S391  D8S1179FGA  D2S1338 
BTR  D2S441  D18S51 FGA     

Technical specifications

 

Investigator ESSplex SE GO! Kit

Investigator ESSplex SE Plus Kit

DNA input volume

1.2 mm FTA punch or 2 µl lysate

Up to 15 µl

Volume per reaction

25 µl

25 µl

Matrix

BT5

BT5

Fluorescence dyes

6-FAM, BTG, BTY, BTO

6-FAM, BTG, BTY, BTO

Genetic Analyzers

ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers

ABI PRISM 310, ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers

Applications
The Investigator ESSplex SE GO! Kit is for rapid and reliable generation of DNA profiles from blood and buccal swabs, and it is highly suited for:

  • Criminal database and forensic reference samples
  • Paternity testing
  • Population genetics studies
  • Other human identity testing applications

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Template Files
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Template File Info Note

 


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Investigator Template Files for GeneMapper ID-X v9 CODIS Software
For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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Investigator Template Files for GeneMapper ID-X v9 Standard Software
For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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Investigator Template Files for GeneMapper ID v6 Software - (EN)

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