For isolation of up to 260 µg total cellular DNA from plant cells and tissues or fungi
- Pure DNA, free from contaminants and enzyme inhibitors
- Rapid isolation of ready-to-use DNA
- No organic extraction, no ethanol precipitation
The DNeasy Plant Maxi Kit provides fast and easy silica-based DNA purification in spin column format. Typical yields are 30–260 μg of high-quality DNA, depending on the sample source.
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DNeasy Plant Maxi Kit (24)
24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)
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68163
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The DNeasy Plant Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
DNeasy Plant and DNeasy 96 Plant procedures.|PCR performance.|PCR analysis.|RAPD analysis.|DNA purity from oak leaves and pine needles.|
|DNA was isolated from arabidopsis leaves using either CTAB lysis (CTAB) or the DNeasy Plant Maxi Kit (DNeasy). Amplification reactions were prepared using purified DNA (1: 50 pg; 2: 100 pg) and primers to the Akin 10 gene. M: markers. (Data kindly provided by Alain Lecharny, Institut de Biotechnologie des Plantes, UMR CNRS-UPS Orsay, France.)|DNA (10 ng) from the indicated leaves or needles was amplified using universal primers for the noncoding intergenic spacer between the tRNA genes trnL (UAA) 5' exon and trnL (UAA) 3' exon of cpDNA (Taberlet, P. et al. (1991) Plant Mol. Biol. 17, 1105). M: 100 bp ladder.|DNA (50 ng) from leaves of the indicated in vitro-propagated sunflower (Helianthus) species was amplified using a 10-base RAPD primer and separated on a 1.5% agarose gel. M: 100 bp ladder. (Data kindly provided by H. J. Henn, Institute of Agricultural Botany, University of Bonn, Germany.)|Spectrophotometric scans (220–320 nm) of DNA isolated from pine needles using the method of Dellaporta, CTAB, or the DNeasy Plant Mini Kit. Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A260/ A280), leading to errors in determination of concentration and purity.|
Performance
The DNeasy Plant Maxi Kit allows rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder Maxi spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.
| Abies alba (silver fir) |
Nicotiana tabacum (tobacco) |
| Aesculus hippocastanum (horse chestnut) |
Oryza sativa (rice)4 |
| Arabidopsis thaliana (thale cress) |
Pelargonium sp. (geranium)4 |
| Avena sp. (oat) |
Petunia sp.4 |
| Brassica napus (oilseed rape) |
Pinus sylvestris (Scotch pine), P. brutia5 |
| Brassica oleracea (kohlrabi) |
Populus tremula (aspen) |
| Chicorium endivia (chicory) |
Pseudotsuga menziesii (Douglas fir) |
| Citrullini lanatus (water melon) |
Quercus robur, Q. petrea (oak)6,7 |
| Egeria sp. |
Rhododendron sp.2,4 |
| Fagus sylvatica (beech)1 |
Rubus idaeus (raspberry) |
| Helianthus spp. (sunflower) |
Solanum tuberosum (potato) |
| Hordeum vulgare (barley)2 |
Sphagnum palustre (moss) |
| Humulus sp. (hops) |
Spinacia oleracea (spinach) |
| Hydrilla sp. |
Taxus baccata (yew) |
| Kalanchoe spp. |
Triticum aestivum (wheat)4 |
| Lupinus sp. |
Ulmus glabra (elm)6 |
| Lycopersicon esculentum (tomato)3 |
Vitis spp. (grape)6 |
| Myriophyllum sp. |
Zea mays (maize) |
The typical yield is 30–260 µg, with a sample size of up to 1 g wet weight, and an elution volume of 500 µl to 2 ml. The DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm (see figures "DNA purity from oak leaves and pine needles"), confirming that the DNA is free of impurities, including enzyme inhibitors. Purified DNA can be used in a wide range of applications (see figures "PCR performance", "PCR analysis", and "RAPD analysis").
Principle
DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary — purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.
Procedure
Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder Maxi spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNeasy Plant Maxi spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.
Applications
The DNeasy Plant Maxi Kit provides purification of DNA from plant tissue, including:
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Plant cells
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Plant tissues
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Fungi
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Feature
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Specifications
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Applications
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PCR, real-time PCR, blotting
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Elution volume
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500 µl – 2 ml
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Format
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Spin column
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Main sample type
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Plant samples
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Processing
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Manual
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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DNA
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Sample amount
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<1 g
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Technology
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Silica technology
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Time per run or per prep
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<2 hours
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Yield
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30–260 µg
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