QIAamp DNA Blood Maxi Kit

For purification of up to 600 µg genomic, mitochondrial, or viral DNA from blood

  • Rapid purification of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Blood Maxi Kit provides silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Maxi Kit is designed for processing 3–10 ml fresh or frozen human whole blood. The QIAamp Maxi spin columns can be easily processed in a centrifuge or on vacuum manifolds (Additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with the vacuum manifolds).

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QIAamp DNA Blood Maxi Kit (10)
For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
 51192
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QIAamp DNA Blood Maxi Kit (50)
For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
 51194
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The QIAamp DNA Blood Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp Spin Column procedure.|Apoptotic banding in stored blood.|Long-range PCR.|Paternity testing by RFLP analysis.|

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. |Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.|Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.|Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed.
(Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)|

Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").

QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table).

DNA yields from human whole blood with different cell densities.
Leukocytes per mlDNA yield (µg) DNA yield (µg)
2.5 x 105 15.8 95.8
1.0 x 106 60.4 91.5
5.0 x 106 312.0 94.5
1.0 x 107 624.6 94.6
Using the QIAamp DNA Blood Maxi Kit, genomic DNA was purified from 10 ml of human whole blood and eluted in 1 ml elution buffer. The first eluate was loaded onto the column a second time and centrifuged again (i.e., re-eluted). Percentage DNA recovery was calculated by assuming that one leukocyte contains 6.6 pg DNA.

Principle
No phenol–chloroform extraction is required. The DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.
Procedure

The QIAamp DNA Blood Maxi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Maxi Kit processes sample sizes of 3–10 ml, with a preparation time of 55 minutes. The typical yield from 10 ml healthy whole blood is 300–600 µg, with an elution volume of 500–2000 µl.

The QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.

Applications

The QIAamp DNA Blood Maxi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Maxi spin columns with 50 ml collection tubes. Sample sources include:

Fresh and frozen whole blood or buffy coat 
Plasma or serum
Bone marrow
Lymphocytes
Body fluids
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Feature
Specifications
Applications PCR, blotting
Elution volume 500–2000 µl
Format Spin column
Main sample type Whole blood, body fluids
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA, viral DNA
Sample amount 3–10 ml
Technology Silica technology
Time per run or per prep 55 minutes
Yield 300–600 µg

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Kit Handbooks
1
QIAamp DNA Blood Midi/Maxi Handbook
For large-scale genomic and viral DNA purification from whole blood, plasma, serum, body fluids, lymphocytes
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References
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