FAQ-1139
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
Since we only use official gene names from Entrez to name our QuantiTect Primer Assays, the name of the assay for one species may differ from that of another species.
For example, interleukin 17 is known as IL-17 in human, and as LOC301289 in rat.
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QuantiTect Primer Assays
QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.