The sequence indicated is the analyzed sequence after bisulfite conversion. Y indicates the potentially methylated sites and boxes indicate the analyzed CpG sites. The asterisk indicates the site for bisulfite conversion control. FP: Forward PCR primer; RPB: Reverse PCR primer (B indicates biotinylation); Seq: Sequencing primer.
Pyrogram trace of unmethylated, bisulfite-converted DNA from a human glioblastoma sample. The bar at dispensation 15 represents the control for completion of bisulfite conversion.