Investigator DIPplex Kit

The Investigator DIPplex Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers. 

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng, and reliable results with <0.1 ng DNA.

The Investigator DIPplex Kit has significant advantages over standard STR- and SNP-based DNA profile generation, including the absence of stutter peaks and the very short amplicons (under 160 bp).

Human identification is commonly based on the analysis of short tandem repeats (STRs) or single nucleotide polymorphisms (SNPs), depending on the demands of an examination or on the sample quality. The Investigator DIPplex Kit uses an alternative means of human identification by allowing the analysis of biallelic deletion/insertion polymorphisms (DIPs, also called INDELs).

The 30 biallelic DIP loci plus Amelogenin are simultaneously amplified in a single PCR and labeled with four dyes. See the table "Technical specifications" for more information.

The Investigator DIPplex Kit is for generation of DNA profiles from forensic stains, hair, and bone samples, and it is highly suitable for:

Disaster victim identification

Anthropological studies

Mixed stain analysis

Other human identity testing applications