Investigator Argus Y-12 QS Kit

For multiplex amplification of 12 Y-chromosomal loci, as recommended by SWGDAM

  • Highly suited for paternity and kinship testing
  • Does not require the isolation of sperm
  • Includes an internal control for PCR efficiency
  • Highly sensitive and inhibitor resistant
  • No overlapping allelic ranges within a panel

The Investigator Argus Y-12 QS Kit is a versatile tool for analyzing Y-chromosomal markers. It is intended for the reliable generation of male DNA profiles from mixtures of male and female DNA without the necessity of separating sperm and female cells. Furthermore, it excellently complements the Investigator Argus X-12 Kit for paternity testing. The kit includes an internal control (Quality Sensor) that provides helpful information on PCR efficiency and the presence of PCR inhibitors in the tested samples. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.

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Investigator Argus Y-12 QS Kit (100)
Primer Mix including an Internal Control (QS), Reaction Mix, Control DNA (XY), Control DNA (XX), DNA Size Standard 550 (BTO), Allelic Ladder Argus Y-12 QS, and Nuclease-Free Water
 383615
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The Investigator Argus Y-12 QS Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.


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Performance

The kit allows the generation of male DNA profiles from mixtures of male and female DNA up to a ratio of 1:4000 so that separation of sperm from female cells or differential lysis is not required.

Generation of DNA profiles using the Investigator Argus Y-12 QS Kit conforms to the guidelines of the International Society for Forensic Genetics. The kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers.

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator Argus Y-12 QS Kit is a multiplex application that amplifies 12 Y-chromosomal STR loci, specifically of the so-called extended minimal haplotype (see table "Chromosomal mapping of the Investigator Argus Y-12 QS Kit"), as recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Developed for use with mixed DNA samples, the Investigator Argus Y-12 QS Kit generates reliable male DNA profiles from DNA mixtures with male to female ratios of up to 1:4000. This makes it unnecessary to separate sperm from female cells or run differential lysis.

The QS in the name stands for Quality Sensor. This special feature is an internal PCR control that provides information on the presence of PCR inhibitors and the efficiency of the PCR.

Chromosomal mapping of the Investigator Argus Y-12 QS Kit
Locus Chromosomal mapping
DYS19 Yp11.2
DYS385 Yq11.222
DYS389-I Yq11.21
DYS389-II Yq11.21
DYS390 Yq11.221
DYS391 Yq11.21
DYS392 Yq11.222
DYS393 Yp11.31
DYS437 Yq11.21
DYS438 Yq11.21
DYS439 Yq11.21
Procedure

The 12 polymorphic Y-chromosomal loci are simultaneously amplified in a single PCR and labeled with the dyes listed in the table "Investigator Argus Y-12 QS Kit dyes and corresponding markers". 

The amplified loci undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

Investigator Argus Y-12 QS Kit dyes and corresponding markers
DyeMarkers
6-FAM DYS439 DYS437 DYS390 DYS385
BTG DYS391 DYS389-I  DYS19 DYS389-II
BTY DYS393 DYS438 DYS392
Technical specifications
Volume per amplification reaction 25 μl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTO
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, and Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator Argus Y-12 QS Kit enables simultaneous amplification of 12 Y-chromosomal STR loci, and it is highly suitable for:

Kinship and paternity testing
Analysis of sexual assault samples
Analysis of forensic stains and samples
Generating male DNA profiles from mixed samples
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Investigator Template Files for GeneMapper ID-X v9 Standard Software
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