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Microbial DNA Positive Control V2 - HC Standard Curve Protocol
Re-Purification of Plasmid DNA Prepared by Methods other than QIAGEN Tips - (EN)
Removal of endotoxins from purified plasmid DNA using the EndoFree® Plasmid Maxi Kit - (EN)
Endotoxin-free DNA is essential for gene therapy research and will improve transfection into sensitive eukaryotic cells.
Isolation of large-construct DNA using the QIAGEN® Plasmid Maxi Kit - (EN)
This protocol is designed to provide up to 150 μg BAC/PAC/P1 DNA or up to 400 μg cosmid DNA.
Filling the chromatography column with QIAGEN® Anion-Exchange Resin (dry powder or slurry) and recycling the column - (EN)
Isolation of single-stranded DNA from M13 phage using QIAGEN® Plasmid Kits - (EN)
Isolation of endotoxin-free plasmid DNA using the QIAGEN® Plasmid Midi Kit - (EN)
This protocol is for purification of up to 100 µg endotoxin-free plasmid DNA using QIAGEN-tip 100.
Isolation of genomic DNA from tissue using the QIAGEN-tip 2500 - (EN)
This protocol is designed for the rapid, easy, and non-toxic preparation of up to 2 mg genomic DNA from not more than 2 g of tissue using QIAGEN-tip 2500. QIAGEN® Genomic-tips 20/G, 100/G, and 500/G can also be used with this protocol by reducing the amount of starting material according to the table on page 2. The purified genomic DNA ranges in size from 50-150 kb.
Isolation of genomic DNA from tissue using the QIAGEN-tip 10000 - (EN)
Sequencing Library Preparation from Cell-Free DNA for Illumina® Sequencing Platforms
Sequencing Library Preparation Using Agencourt® AMPure® XP Beads to Remove Adapter Dimers - (EN)
Fast-forward protocol for transient transfection of NIH/3T3 cells in 96-well plates using PolyFect® Transfection Reagent - (EN)
Fast-forward protocol for transient transfection of HeLa-S3 cells in 96-well plates using PolyFect® Transfection Reagent - (EN)
Fast-Forward cotransfection of adherent cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent - (EN)
Fast-forward protocol for transient transfection of CHO cells in 96-well plates using PolyFect® Transfection Reagent - (EN)
Fast-forward protocol for transient transfection of CHO cells in 96-well plates using Effectene® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of CHO cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.
Fast-forward protocol for transient transfection of COS-7 cells in 96-well plates using PolyFect® Transfection Reagent - (EN)
Fast-forward protocol for transient transfection of HeLa cells in 96-well plates using PolyFect® Transfection Reagent - (EN)
Fast-forward protocol for transient transfection of 293 cells in 96-well plates using Effectene® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of 293 cells in 96-well plates without pre-plating of cells 24 h prior to transfection. Cell plating and transfection are performed on the same day, making this protocol rapid and convenient.
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