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Lysis time will vary depending on the size and density of the source material. The QIAGEN Genomic-tip will run slowly due to the high debris content in the nuclear fraction. The yield is about 0.3-0.4 µg DNA/fly.
The expected yield of genomic DNA from 0.5–2.5 ml of clotted whole blood should be in the range of 8–40 μg.
The procedure has been successfully used for isolation of mRNA from adipose tissue by customers. Because of the nature of adipose tissue it cannot be used immediately in Oligotex Direct mRNA Protocols; simple centrifugation, however, eliminates the problem, and the procedure works fine.
The procedure has been used successfully by customers for regeneration of Oligotex resin from spin columns following elution of poly A+ mRNA.
This procedure has been developed by customers for the transient transfection of HEK 293 cells in 75 cm3 flasks using QIAGEN® PolyFect® Transfection Reagent. It has not been thoroughly tested and optimized by QIAGEN.
The protocol has been used successfully for Cy3-, Cy5-, and biotin-labeling of cDNA from <50 ng of total RNA or poly A+ mRNA.
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