Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

RT² SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT² qPCR Primer Assays, RT² Profiler PCR Arrays, RT² lncRNA qPCR Assays, and RT² lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT² SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.

RT² Profiler PCR Arrays enable quick, reliable gene expression analysis of 170+ pathways, allowing researchers to spend less time at the bench and more time interpreting results. The arrays are pathway-focused panels of laboratory-verified qPCR assays, with integrated, patented controls to ensure a successful experiment every time. PhD-trained application specialists are available to provide technical support for the arrays, and each array can also be modified to suit unique experimental needs. For biomarker discoveries, we offer Pathway Plus PCR arrays and all arrays listed below for the Fluidigm BioMark Real-Time PCR System.

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.