FAQ-1745
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
CoralLoad dyes supplied in PCR Kits such as, e.g., Taq, HotStarTaq, and TopTaq DNA Polymerase and TopTaq Master Mix do not interfere with most downstream enzymatic applications.
However, for reproducible results, purification of PCR products using the QIAquick or MinElute PCR Purification Kits prior to enzymatic manipulation is recommended.
Related products
Taq DNA Polymerase
Taq DNA Polymerase is a replicative polymerase derived from the thermophilic eubacterium Thermus aquaticus. Thermostable activity at temperatures above 70°C makes the enzyme suitable for standard and specialized PCR amplification applications. QIAGEN Taq DNA Polymerase is supplied with PCR Buffer specially formulated for fast setup with minimal optimization of PCR parameters, Q-Solution to facilitate amplification of "difficult" (e.g., GC-rich) templates and the additional time-saving advantage of CoralLoad PCR Buffer with two gel-tracking dyes to enable immediate loading of PCR products.
QIAGEN Taq DNA Polymerase is also available in Taq PCR Core Kit that includes dNTPs. If you prefer a Master Mix format, including all needed components, go to Taq PCR Master Mix Kit.
The source of Taq DNA polymerase, the thermophilic eubacterium Thermus aquaticus, was first identified in 1966, living and thriving at about 75°C in the waters of a hot spring in Yellowstone National Park. Taq DNA polymerase has a temperature optimum of 72°C and does not denature at 95°C. This intrinsic thermostability makes Taq DNA polymerase suitable for standard and specialized PCR amplification applications based on its core competence to maintain relatively high catalytic activity and stability after multiple rounds of thermal cycling at high temperatures.
Taq DNA polymerase has 5’→3’ exonuclease activity to remove RNA primers but lacks 3’→5’ exonuclease “proofreading” activity. Many DNA polymerases perform highly accurate DNA synthesis even in the absence of exonucleolytic proofreading. In nature, Thermus aquaticus compensates for the lack of proofreading with a mismatch repair (MMR) system including a MutS homolog that plays a crucial role in correcting replication errors. Taq DNA polymerase performs best when amplifying DNA fragments <2 kb but can amplify longer fragments efficiently under defined reaction conditions — dNTP concentration, pH and the concentration of MgCl2 relative to the total concentration of dNTPs present. Under these conditions, an error rate for Taq DNA polymerase per nucleotide polymerized at 70°C can be achieved as low as 10-5 for base substitution errors and 10-6 for frameshift errors.
The non-template-dependent terminal transferase activity inherent in Taq DNA polymerase and other nonproofreading DNA polymerases provides a highly efficient method to clone PCR products. Taq DNA polymerase adds a single, unpaired residue, preferentially an adenosyl residue, to each 3'-end of a double-stranded amplified product (extra A addition). This property is an advantage for the TA-cloning strategy but may present drawbacks when Taq DNA polymerase is used for microsatellite genotyping analysis.
QIAGEN Taq DNA Polymerase is designed to deliver fast, reliable PCR-related workflows that are easily optimized for efficient amplification performance and reduced error rates.
If you are looking for a DNA polymerase with higher fidelity or longer range than Taq DNA Polymerase, explore our long range or higher fidelity enzymes and mixes. Long range PCR provides highly sensitive and specific long-range amplification for up to 30 kb using any DNA or cDNA template. High fidelity enzymes and master mixes confer higher fidelity, speed and performance compared to standard Taq DNA Polymerase.
QIAquick PCR Purification Kit
The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick PCR Purification standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAquick PCR Purification protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.
MinElute PCR Purification Kit
The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
HotStarTaq DNA Polymerase
HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. HotStarTaq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided.