FAQ-2060
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
The Type-it Microsatellite PCR Kit was optimzed and validated for this size range since microsatellite analysis typically only uses fragments of up to 500 bp in length.
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Type-it Microsatellite PCR Kit
The Type-it Microsatellite PCR Kit is based on highly specific HotStarTaq Plus DNA Polymerase and a patented buffer system, both of which enable reliable multiplex PCR-based microsatellite analysis without optimization. The combination of all components provided in the master mix and the specialized formulation result in highly specific amplification of all loci in parallel. The kit is dedicated to fast and reliable microsatellite analysis by multiplex PCR. Optimized protocols are also provided to enable subsequent analysis either by high-resolution capillary sequencing or by using other electrophoresis instruments.