| Full name |
Purification of total RNA from animal tissues for microarray analysis (aqueous phase) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_RNeasyMicroarrayTissue_AnimalTissues_AqueousPhase_V1.pdf (PDF 76 KB)
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| Description |
This protocol is for purification of total RNA from up to 100 mg frozen or 50 mg stabilized animal tissue using the RNeasy Microarray Tissue Mini Kit. RNA is purified from the aqueous phase of tissue homogenates treated with chloroform. |
| Protocol Version |
1 |
| Sample size |
Up to 100 mg frozen or 50 mg stabilized tissue disrupted in 1 ml QIAzol Lysis Reagent |
| Elution volume |
30-100 µl in increments of 10 µl; default 50 µl |
| Applications |
RNA |
| Starting material |
Tissue, Animal tissues |
| Kits |
RNeasy Microarray Tissue Mini Kit
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