| Full name |
RNA Purification from blood with QIAshredder homogenization and on-column DNase digest |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_QIAampRNABloodMini_350ulLysedLeukocytes_DNaseDigest_V2.pdf (PDF 83 KB)
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| Description |
This protocol is for purification of RNA from 350 µl lysed leukocytes using the QIAamp RNA Blood Mini Kit. On-column DNase digestion using the RNase-Free DNase Set provides efficient digestion of DNA during RNA purification. |
| Protocol Version |
2 |
| Sample size |
Up to 2 x 10e6 leukocytes lysed in 350 µl Buffer RLT |
| Elution volume |
Elution 1: 30 - 50 µl in increments of 5 µl; elution 2: 20 - 50 µl in increments of 5 µl |
| Applications |
RNA |
| Starting material |
Blood, Leukocytes |
| Kits |
QIAamp RNA Blood Mini Kit
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