| Full name |
Purification of bacterial or yeast DNA with enzymatic lysis |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_DNA_DNeasyBloodAndTissue_Bacteria_Gram-positive_OrYeast_EnzymaticLysis_V1.pdf (PDF 80 KB)
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| Description |
This protocol is for purification of bacterial or yeast DNA using the DNeasy Blood & Tissue Kit. Gram-positive bacteria or yeast cells are lysed enzymatically. DNA is then purified using a fully automated protocol. |
| Protocol Version |
1 |
| Sample size |
Bacteria or yeast cells resuspended in 180 µl enzyme solution |
| Elution volume |
Elution volume 1: 50 µl; elution volume 2: 50 - 250 µl in increments of 25 µl; default: 150 µl |
| Applications |
DNA |
| Starting material |
Bacteria (Gram+) or yeast |
| Kits |
DNeasy Blood & Tissue Kit
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