| Full name |
Purification of total RNA from up to 5 mg animal tissue or up to 5x10e5 cells with DNase digest |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_RNeasyMicro_AnimalTissuesAndCells_DNaseDigest_V2.pdf (PDF 89 KB)
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| Description |
This protocol is for purification of concentrated total RNA from small amounts of tissue or small numbers of cells using the RNeasy Micro Kit. On-column DNase digestion provides efficient digestion of DNA during RNA purification. |
| Protocol Version |
2 |
| Sample size |
Up to 5 mg fresh/frozen tissue, 10 mg stabilized tissue, or up to 5x10e5 cells disrupted in RLT |
| Elution volume |
14-30 µl in increments of 1 µl, default 15 µl |
| Applications |
RNA |
| Starting material |
Animal tissues and cells |
| Kits |
RNeasy Micro Kit
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