| Full name |
Purification of large RNAs and miRNA-enriched fractions (aqueous phase) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_miRNeasyMini_AnimalTissuesAndCells_AqueousPhasePartAandB_V2.pdf (PDF 146 KB)
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| Description |
This protocol is for purification of large and small (miRNA-enriched) RNA fractions from up to 50 mg frozen or 25 mg stabilized tissue, or up to 1 x 10e7 cells using the miRNeasy Mini Kit. RNA is purified from the aqueous phase of tissue or cell homogenates after organic extraction. The protocol consists of 2 parts: part A (purification of large RNAs) and part B (purification of small RNAs). |
| Protocol Version |
2 |
| Sample size |
Aqueous phase from up to 50 mg frozen or 25 mg stabilized tissue, or up to 1 x 10e7 cells |
| Elution volume |
large RNAs: 30 - 100 µl in increments of 10 µl; miRNAs: 20 µl |
| Applications |
RNA |
| Starting material |
Animal cells, Animal tissues and cells, Tissue, Animal tissues |
| Kits |
miRNeasy Mini Kit
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