| Full name |
Purification of miRNA-enriched fractions separate from larger RNAs (aqueous phase) |
| Protocol Download |
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|
| Protocol Sheet Download |
ProtocolSheet_RNA_miRNeasyMini_AnimalTissuesAndCells_AqueousPhasemiRNA_V2.pdf (PDF 101 KB)
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| Description |
This protocol is for purification of miRNA-enriched fractions separate from larger RNAs using the miRNeasy Mini Kit. RNA is purified from the aqueous phase of tissue or cell homogenates treated with chloroform. |
| Protocol Version |
2 |
| Sample size |
Aqueous phase from up to 50 mg frozen or 25 mg stabilized tissue, or up to 1 x 10e7 cells |
| Elution volume |
20 µl |
| Applications |
RNA |
| Starting material |
Animal tissues and cells |
| Kits |
miRNeasy Mini Kit
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