| Full name |
Purification of total RNA from animal cells (QIAshredder homogenization and on-column DNase digest) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_RNeasyMini_AnimalCells_QIAshredderDNaseDigest_V1.pdf (PDF 89 KB)
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| Description |
This protocol is for purification of total RNA from animal cells using the RNeasy Mini Kit. The protocol includes QIAshredder homogenization and on-column DNase digest. |
| Protocol Version |
1 |
| Sample size |
5x10e6 - 1x10e7 cells disrupted in 600 µl Buffer RLT |
| Elution volume |
30-100 µl in increments of 10 µl; default 50 µl |
| Applications |
RNA |
| Starting material |
Animal cells |
| Kits |
RNeasy Mini Kit
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