| Full name |
Purification of total RNA from 5 x 10e6 - 1 x 10e7 animal cells (with QIAshredder homogenization) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_RNeasyMini_AnimalCells_QIAshredder_V3.pdf (PDF 88 KB)
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| Description |
This protocol is for purification of total RNA up to 1 x 10e7 animal cells using the RNeasy Mini Kit. Disrupted samples are homogenized using a QIAshredder spin column. |
| Protocol Version |
3 |
| Sample size |
5 x 10e6 - 1 x 10e7 cells disrupted in 600 µl Buffer RLT |
| Elution volume |
30-100 µl in incements of 10 µl; default 50 µl |
| Applications |
RNA |
| Starting material |
Animal cells |
| Kits |
RNeasy Mini Kit
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