| Full name |
Purification of total RNA, including small RNAs, from animal tissues and cells (aqueous phase) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_miRNeasyMini_AnimalTissuesAndCells_AqueousPhase_V2.pdf (PDF 92 KB)
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| Description |
This protocol is for purification of total RNA, including miRNA and other small RNA molecules, from up to 50 mg frozen or 25 mg stabilized tissue, or up to 1 x 10e7 cells using the miRNeasy Mini Kit. RNA is purified from the aqueous phase of tissue or cell homogenates treated with chloroform. |
| Protocol Version |
2 |
| Sample size |
Up to 50 mg frozen or 25 mg stabilized tissue, or up to 1 x 10e7 cells disrupted in QIAzol |
| Elution volume |
30–100 µl in increments of 10 µl; default 50 µl |
| Applications |
RNA |
| Starting material |
Animal tissues and cells |
| Kits |
miRNeasy Mini Kit
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