| Full name |
6xHis-tagged protein purification under native conditions |
| Protocol Download |
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|
| Protocol Sheet Download |
ProtocolSheet_Protein_NiNTASpin_UpTo5mlEcoliCultures_Native_V3.pdf (PDF 83 KB)
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| Description |
This protocol is for purification of up to 300 µg 6xHis-tagged protein from bacterial pellets under native conditions using the Ni-NTA Spin Kit. |
| Protocol Version |
3 |
| Sample size |
Up to 5 ml E. coli cultures |
| Elution volume |
300 µl |
| Applications |
Protein |
| Starting material |
E. coli cultures, Up To 5 ml LB cultures |
| Kits |
Ni-NTA Spin Kit
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