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Information about QIAcube Standard Protocol: QIAampMinEluteCcfDNA PlasmaOrSerum Standard
Full name
Isolation of ccfDNA from plasma
Protocol Download
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Protocol Sheet Download
ProtocolSheet_Cleanup_QIAampMinEluteCcfDNA_PlasmaOrSerum_Standard_V1.pdf
(PDF 476 KB)
Description
This protocol is for the cleanup of circulating DNA following the pre-concentration steps of the QIAamp MinElute ccfDNA Mini or Midi kit. Prior to processing on the QIAcube pre-concentration of up to 10 ml plasma or serum must be performed. Resulting ccfDNA is then purified using a fully automated protocol.
Protocol Version
1
Sample size
200 µl
Elution volume
Elution Volume: 20–80 µl in increments of 5 µl, default set to 30 µl
Applications
Cleanup
Starting material
Plasma or serum
Kits
QIAamp MinElute ccfDNA Midi Kit
QIAamp MinElute ccfDNA Mini Kit
ProtocolView