| Full name |
Purification of total RNA from animal tissues and cells (with 2 elution steps) |
| Protocol Download |
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| Protocol Sheet Download |
ProtocolSheet_RNA_RNeasyMini_AnimalTissuesAndCells_TwoElutionSteps_V2.pdf (PDF 74 KB)
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| Description |
This protocol is for purification of total RNA from up to 20 mg frozen or 10 mg stabilized easy-to-lyse tissue, or up to 5 x 10e5 animal cells using the RNeasy Mini Kit. The second elution step increases yields of RNA. |
| Protocol Version |
2 |
| Sample size |
Up to 20 mg frozen or 10 mg stabilized tissue, or up to 5 x 10e5 cells disrupted in 350 µl RLT |
| Elution volume |
Elution volume 1 and 2: 30–100 µl in increments of 10 µl; default 50 µl |
| Applications |
RNA |
| Starting material |
Animal cells, Animal tissues and cells, Tissue, Animal tissues |
| Kits |
RNeasy Mini Kit
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