The first lane shows the DNA size standard (DNA-Ladder). Sample 1 is negative. Sample 2 is positive for TWIST1, sample 3 is positive for Akt-2 and PI3Kα, and sample 4 is positive for PI3Kα and TWIST1. Signals for all three EMT-2 markers can be detected in sample 5 and actin is detected in samples 1 to 5. The PCR negative control (C-) and the positive control (EMT-2) are shown in the last two lanes.
In lung cancer, the AdnaTest CTC-Select provides antibodies against EpCam, Her2, EGFR, resulting in a strong increase of performance compared to detection based only on EpCam (Hanssen et al. (2016) Nature Sci Rep. 6, 28010).
The first lane shows the DNA size standard (DNA-Ladder). Samples 1 is positive for ALDH1 and GA733.2, sample 3 is positive for ALDH1 and sample 2 is negative. The PCR negative control and the positive control (LungDetect) are shown in the last two lanes.