AdnaTest ProstateCancerPanel AR-V7
For the enrichment and molecular characterization of circulating tumor cells (CTCs) from whole human blood
AdnaTest ProstateCancerPanel AR-V7 allows molecular characterization of CTCs using the Combination of Combinations Principle (COCP). AdnaTest ProstateCancerPanel AR-V7 is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood. This allows sensitive analysis of prostate cancer-associated gene expression including AR-V7 detection in immunomagnetically enriched tumor cells by reverse transcription and PCR.
The AdnaTest ProstateCancerPanel AR-V7 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The AdnaTest’s successful CTC detection is based on the Combination of Combinations Principle (COCP). Each AdnaTest has a unique combination of tumor associated markers and an optimized combination of antibodies for cell selection. The combination of highly specific immunomagnetic cell selection system (using the optimized antibody combination, see “A CTC captured by three antibodies coupled to magnetic beads”) and highly sensitive RT-PCR technology (using a combination of mRNA tumor markers) provides the highest degrees of analytical specificity and sensitivity.
The AdnaTest uses a two-step process (Select and Panel) to generate results within 6 hours (see “AdnaTest ProstateCancerSelect: Immunomagnetic cell selection with multiple tumor associated antibodies” and “AdnaTest ProstateCancerPanel AR-V7: qRT-PCR of AR-V7 and various prostate cancer associated tumor markers.”).
AdnaTest ProstateCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor associated antigens. Antibodies against these antigens are conjugated to magnetic beads for labeling of tumor cells in whole blood. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S) and are subsequently lysed (“AdnaTest ProstateCancerSelect: Immunomagnetic cell selection with multiple tumor associated antibodies.”). The cell lysate is used for further analysis with AdnaTest ProstateCancerPanel AR-V7.
AdnaTest ProstateCancerPanel AR-V7 contains Oligo (dT)25 Beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. Reverse transcription results in cDNA, which is subsequently used as template for preamplification and tumor cell detection and characterization by qRT-PCR. The AdnaPanel PrimerMixes allow amplification of four tumor associated antigens and two control genes. Furthermore, the kit contains PrimerMixes for amplification of an internal control and an inhibition control. See "AdnaTest ProstateCancerPanel AR-V7: qRT-PCR of AR-V7 and various prostate cancer associated tumor markers."
qRT-PCR linearity and efficiency
Field test results
fragment fix placeholder