MagAttract Microbial DNA Kit

For the automated isolation of DNA from microbial and food cultures

Features

  • Quick processing of samples using SwiftMag technology
  • Efficient Inhibitor Removal Technology removes PCR inhibiting compounds
  • High-quality DNA isolation from various microbial cultures using automation
MagAttract Microbial DNA Kit (384)

Cat. No. / ID: 27200-4

For the automated isolation of DNA from microbial and food cultures
Kit
MagAttract
Beads
The MagAttract Microbial DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The MagAttract Microbial DNA Kit is designed for isolating DNA from up to 1.8 ml of culture with automated, hands-free systems. It allows for the identification of DNA from a range of microorganisms found in food and other samples. The MagAttract Microbial DNA Kit includes Inhibitor Removal Technology to remove compounds that inhibit downstream processes, including polysaccharides and lipids, leaving high-quality DNA for qPCR, Sanger sequencing and next-generation sequencing applications. Bacteria and fungi can be identified from meats, dairy products, fruits, vegetables and chocolate.

The MagAttract Microbial DNA Kit was created with automated processing and liquid handling systems in mind. QIAGEN offers protocols for the Thermo Scientific KingFisher and Eppendorf epMotion platforms, but other systems, such as those offered by Tecan, Hamilton and Beckman are also available.

Tools to complete your workflow:

The MagAttract Microbial DNA Kit was previously sold by MO BIO as the PowerMag Microbial DNA Kit.

Specifications

FeaturesSpecifications
sampleamount<1 g
applicationsPCR, real-time PCR, blotting
purificationoftotalrnamirnapolyamrnadnaorproteinDNA
samplesize1.8 ml
throughput96-well plate
formatSwiftMag Technology
sampletypesProcessed microbial cultures, food cultures and swabs
storagetemperatureRNase A should be stored at 4°C The other kit reagents and components should be stored at room temperature (15-30°C)
beadsize0.1 mm glass

Resources

Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699