DNeasy PowerWater Kit

For the isolation of genomic DNA from filtered water samples, including turbid water

Features

  • Highly pure genomic DNA isolation free from organic materials
  • Optimized to increase DNA yields from low biomass samples
  • Compatible with most common filter membrane types
  • Easily removes blocks to downstream PCR with Inhibitor Removal Technology
DNeasy PowerWater Sample (2)

Cat. No. / ID: 14900-S

For the isolation of genomic DNA from filtered water samples, including turbid water.
KitTube
DNeasy PowerWater Kit
PowerWater DNA Bead Tube
Preparations
2
100
50
The DNeasy PowerWater Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Using Inhibitor Removal Technology, the DNeasy PowerWater Kit isolates genomic DNA from filtered water samples, free from salts, metals, humic substances and other organic materials. The kit can isolate high-quality DNA even from water containing high levels of contaminants. Resulting DNA can be used in any downstream application, including qPCR, Sanger sequencing and next-generation sequencing. Viable DNA can be acquired from common filter membrane types, including 0.45 μm and 0.22 μm filter funnels. DNA is ready to use in a final 100 μl volume. Want to try this solution for the first time? Request a quote for a trial kit. Purification of DNA using the DNeasy PowerWater Kit can be automated on the QIAcube Connect.

The DNeasy PowerWater Kit was previously sold by MO BIO as the PowerWater DNA Isolation Kit.

Specifications

FeaturesSpecifications
formatSilica Spin Filter Tubes
sampletypesOcean water, fresh water, brackish water, ground water, waste water
throughput1-6 samples
timeperrunorperprep20 minutes
processingBead beating
bindingcapacityUp to 20 µg per prep
storagetemperatureStore at room temperature (15-30°C)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699