DNeasy PowerLyzer Microbial Kit

For the bead-based isolation of high-quality DNA from microbial cultures

Features

  • Short DNA isolation protocol in just 20 minutes from yeast, fungi and bacterial cultures
  • Compatible for use with the PowerLyzer 24 Homogenizer and other bead-based homogenizers
  • Ready-to-use, highly pure DNA for downstream applications
DNeasy PowerLyzer Microbial Kit (50)

Cat. No. / ID: 12255-50

For the bead-based isolation of high-quality DNA from microbial cultures
Preparations
50
2
The DNeasy PowerLyzer Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Isolate high-quality genomic DNA from up to 1.8 ml of microbial culture with the DNeasy PowerLyzer Microbial Kit.

 

This kit successfully tests a variety of microorganisms, including Gram (+/-) bacteria, yeast and spores, by lysing microorganisms through a combination of heat, detergent and mechanical 0.1 glass bead digestion. The PowerLyzer 24 Homogenizer is recommended for this kit.

DNeasy PowerLyzer Microbial Kit was formerly sold by MO BIO as PowerLyzer Ultraclean Microbial DNA Isolation Kit.

Performance



Supporting data and figures

Specifications

FeaturesSpecifications
formatSilica Spin Filter Tubes
samplesize1.8 ml
throughput1-24 samples
bindingcapacityUp to 20 µg per prep
processingBead beating
timeperrunorperprep20 minutes
storagetemperatureStore at room temperature (15-30°C)
beadsize0.1 mm glass
sampletypesProcessed cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi

Resources

Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699