AllPrep DNA/RNA Micro Kit

For simultaneous purification of DNA and RNA from small cell and tissue samples
  • High-quality DNA and RNA from the same sample
  • Maximal yields of DNA and RNA from precious samples
  • Rapid purification with short, streamlined protocol
  • Ready-to-use DNA and RNA for any downstream analysis

The AllPrep DNA/RNA Micro Kit allows the simultaneous purification of genomic DNA and total RNA from the same biological sample. DNA is purified using the novel AllPrep DNA spin column. RNA is purified from the AllPrep column flow-through using an RNeasy MinElute spin column. The kit is compatible with up to 5 x 105 cells or 5 mg tissue. It can be automated on the QIAcube Connect.

产品 货号 目录价:
AllPrep DNA/RNA Micro Kit
For 50 micropreps: AllPrep DNA Spin Columns, RNeasy MinElute Spin Columns, Collection Tubes, Carrier RNA, RNase-Free Water and Buffers
80284
询价

AllPrep DNA/RNA Micro Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


  • Main Image Navi
性能

Both cultured cells and easy-to-lyse tissues can be processed with the AllPrep DNA/RNA Micro Kit (see table). Genomic DNA is purified using the novel AllPrep DNA spin column, and total RNA is purified using an RNeasy MinElute spin column. Total RNA with Agilent RIN values of close to 10 is routinely obtained from cultured cells. The purified genomic DNA has an average length of 15–30 kb depending on homogenization conditions (see figure "Simultaneous purification of total RNA and genomic DNA"). Genomic DNA and total RNA show reliable quantification over a wide dynamic range (see figure "Wide dynamic range").  

Optimal yields of DNA and RNA from tissues
Tissue (5 mg)DNA yield (µg)* RNA yield (µg)*
Heart 3.89 2.00
Brain 2.60 3.08
Kidney 11.33 12.70
Liver 32.40 16.02
Lung 9.65 24.63
Spleen 20.50 43.51
Thymus 34.90 56.89
Intestine 26.71 27.38
* DNA and RNA were purified from two 5 mg samples of each tissue, and the average yields are shown.

原理

The AllPrep DNA/RNA Micro Kit is designed for purifying both genomic DNA and total RNA from a single cell or tissue sample, and is highly suited for small samples such as laser-microdissected cryosections and fine-needle aspirates. Since there is no need to divide the sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application.

操作流程

A simple workflow allows the purification of high-quality DNA and RNA from the same sample in as little as 40 minutes (see flowchart "AllPrep DNA/RNA procedure"). Both cultured cells and easy-to-lyse tissues can be processed. Genomic DNA is purified using the novel AllPrep DNA spin column, and total RNA is purified using an RNeasy MinElute spin column.

When disrupting and homogenizing tissues in Buffer RLT Plus (supplied with the AllPrep DNA/RNA Micro Kit), excessive foaming may occur. This foaming is substantially reduced by adding Reagent DX (supplied separately) to Buffer RLT Plus at a final concentration of 0.5% (v/v) before starting disruption and homogenization. Reagent DX has been carefully tested with the kit, and has no effect on RNA purity or on downstream applications such as real-time RT-PCR. Buffer RLT Plus containing Reagent DX can be stored at room temperature (15–25ºC) for at least 9 months.

A supplementary protocol allows optional purification of protein. For experiments with larger samples, the AllPrep DNA/RNA/Protein Mini Kit can be used to simultaneously purify DNA, RNA, and protein from the same cell or tissue sample.

应用

The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is suited for any application, including Southern-, dot-, and slot-blot analyses; and PCR and multiplex PCR.

The purified total RNA can be used in any application, such as RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.

A supplementary protocol allows optional purification of protein. The purified protein is denatured and can be used in applications such as SDS-PAGE, western blotting, and 2D gel electrophoresis.

特点
参数
应用 PCR, RT-PCR, real-time PCR, cDNA synthesis, microarray, blotting
洗脱体积 DNA: 30 µl; RNA: 10 µl
规格 Spin column
主要样本类型 Cells, tissue
处理 Manual (centrifugation)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA and total RNA
样本量 5 x 10e5 cells or 5 mg tissue
技术 Silica technology
每次运行或制备时间 35 min
产量 Varies

您无权限查看此资源

试剂盒操作手册
1
For simultaneous purification of genomic DNA and total RNA from the same small sample, including
  • animal and human cells (≤5 x 10^5)
  • animal and human tissues (≤5 mg)
  • microdissected cryosections

显示更多
安全数据表
1
Download Safety Data Sheets for QIAGEN product components.
浏览
图片