Ni-NTA HisSorb Strips
For assays using His-tagged biomolecules
Ni-NTA Strips are 8-well strips whose wells are coated with Ni-NTA. They are used for immobilization of His-tagged proteins in protein interaction assays, such as ELISA.
Ni-NTA HisSorb Strips are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Principle
Ni-NTA HisSorb Strips provide the power of the QIAexpress Ni-NTA System in solid-phase 8-well format for convenient assay and screening of His-tagged biomolecules, even if crude lysates. Ni-NTA moieties, which coat the entire surface of each well, deliver effective, directed immobilization of functionally active 6xHis-tagged molecules, leading to more reliable and reproducible assay results and higher signal-to-noise ratio than are possible with other systems.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
Ni-NTA HisSorb Strips are suitable for a range of applications, including:
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