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DNeasy UltraClean Microbial Kit

For isolation of high quality DNA from microbial cultures

  • Optimized to isolate DNA from yeast, bacteria or fungi
  • High-quality DNA ready for downstream applications
  • Fast protocol yields pure DNA in just 20 minutes
When clean DNA is what you need, the DNeasy UltraClean Microbial Kit delivers, isolating high-quality genomic DNA from 1.8 ml of microbial culture. Beginning with a bead-beating protocol, cells are lysed through a combination of mechanical force, heat and detergent, vortexed using our specially designed adapter for the Vortex Genie 2 Vortex, bound to a silica spin filter and washed. The resulting DNA is recovered in a DNA-free Tris buffer.

DNeasy UltraClean Microbial Kit was formerly sold by MO BIO as UltraClean Microbial DNA Kit.
Cat No./ID: 12224-250
DNeasy UltraClean Microbial Kit (250)
Order Product
For isolation of high quality DNA from microbial cultures
Cat No./ID: 12224-50
DNeasy UltraClean Microbial Kit (50)
Order Product
For isolation of high quality DNA from microbial cultures
The DNeasy UltraClean Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Figure 1. High quality DNA purified from a variety of microorganisms
Lane 1: 1 kb ladder
Lane 2: E. coli
Lane 3: P. aeruginosa
Lane 4: S. aureus
Lane 5: M. luteus
Lane 6: B. subtilis
Lane 7: B. pumulis
Lane 8: E. faecalis
Lane 9: L. casei
Lane 10: 1 kb ladder
Lane 11: A. niger
Lane 12: C. albicans
Lane 13: S. cerevisiae
Lane 14: S. cerevisiae

Figure 1. Analysis displayed on 1.2% TAE agarose gel. Lower yields obtained with A. niger (11) and C. albicans (12) due to lower cell densities in starting culture.

Bead size 0.15 mm garnet
Binding capacity Up to 20 µg per prep
Format Silica Spin Filter Tubes
Processing Bead beating
Sample size 1.8 ml
Sample types Processed cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 30 minutes
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