miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced miRNA qPCR
miRCURY LNA miRNA Focus PCR Panels enable exceptionally sensitive and specific miRNA profiling with the miRCURY LNA miRNA PCR System. These PCR primer panels are enhanced with LNA technology and are specific for particular sample types, diseases or pathways, providing a flexible solution that allows you to focus on the miRNAs relevant to your application. miRCURY LNA miRNA Focus PCR Panels are pre-aliquoted in 96- or 384-well PCR plates and are ready for use.
Serum/Plasma miRNA PCR PanelsWhy study miRNAs in serum and plasma?
miRNAs are present in small amounts in serum and plasma. They circulate in the blood in a highly stable, cell-free form, suggesting that they are protected from the RNA-degrading enzymes found in blood (see figures miRNAs are stable in serum and plasma and Complete miRNA serum profile).
miRNA profiles have been shown to be altered in serum and plasma taken from patients suffering from cancer and other diseases. This makes miRNAs promising candidates for use in non-invasive discovery of important new biomarkers for a wide range of diseases and biological processes. Blood samples are easy to obtain and carry little risk to the patient, compared with biopsies and surgical procedures.
Challenges of miRNA profiling in serum and plasma
Serum and plasma only contain a fraction of the RNA found in whole blood (see figure Blood serum and plasma contain small amounts of RNA). Most of the RNA is found in the buffy coat, which mainly consists of white blood cells and platelets. To get accurate miRNA profiles, it is important to ensure that the serum/plasma is not contaminated by RNA from blood cells, as this would skew the results of the experiment.
Several other challenges exist when profiling miRNAs in serum and plasma, including:
Overcoming these challenges requires an extremely accurate miRNA qPCR system. The miRCURY LNA miRNA PCR system has a proven track record for reliable detection of miRNAs in serum and plasma samples.
Cancer Focus miRNA PCR PanelsmiRNA and cancer
Numerous studies have demonstrated links between miRNA dysregulation and various disease states, including a diverse array of human cancers. miRNAs have been shown to act as both oncogenes and tumor suppressors. The high relative stability of miRNA in common clinical source materials (e.g., FFPE blocks, plasma, urine, saliva, etc.) and the ability of miRNAs to function as molecular markers for discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of applications. miRNAs can be used as disease classifiers for different types of cancers or as biomarkers to monitor the effects of therapy on cancer prognosis and progression.
Identify miRNA biomarkers or new classifiers
Cancer Focus miRNA PCR Panels can be used for many types of cancer miRNA profiling from samples including FFPE and biofluids. These miRNA profiles can be used to identify unique cancer signatures and help identify biomarkers or classifiers for cancer types and progression (see figures Six types of cancers compared and miRNA expression from four types of cancer).
Exosome Focus miRNA PCR PanelsHow the assays were selected
All miRNA assays in the Exosome Focus miRNA PCR Panels were selected based on a combination of experimental data from in-house studies on exosomes using our miRCURY LNA miRNA PCR System and results published in peer-reviewed journals. The assays are not a complete list targeting all miRNAs found in exosomes, but they target those miRNAs typically found (see figure Urine and CSF miRNA profiles). If missing some miRNAs is a concern, we recommend running a full miRNome study using the miRCURY LNA miRNA miRNome PCR Panels.
Serum/Plasma miRNA PCR PanelsProduct coverage
All 179 assays on the Serum/Plasma miRNA PCR Panels have been carefully selected based on our vast number of in-house analyses of miRNA expression in blood, serum and plasma samples, as well as on the limited number of peer-reviewed published papers available. For a complete list of miRNA assays on the panel, refer to the plate layout file.
Over 1 million in-house and collaborative data points from serum/plasma samples collected from healthy and diseased individuals have been used in the selection of relevant miRNAs for the panel. This includes miRNA expression data from different stages of various cancers, neurological disorders, neurodegenerative diseases, allergies, inflammation, etc. Our database is largely based on total miRNA extracted from serum/plasma, so a particular circulating miRNA should be on the focus panel, regardless of whether it is preferentially protein bound, found in exosomes, microvesicles or otherwise compartmentalized. This results in a very comprehensive panel with broad applications.
The Serum/Plasma miRNA PCR Panels are available in two formats:
Finding good reference genes in serum and plasma samples can be challenging. Some of the common house-keeping genes, such as U6 are not present in these samples, and the overall RNA concentration is low. The Serum/Plasma Focus miRNA PCR Panels contain 7 potential reference genes:
Cancer Focus miRNA PCR PanelsThe miRCURY LNA miRNA Cancer Focus PCR Panel targets 84 human miRNAs related to cancer that have been shown to be important for tumorigenesis and in cancer diagnostics. These miRNAs can be used as markers for 27 different types of cancer. They have been identified from a large biobank containing thousands of FFPE samples, as well as from literature data. For a list of references of each miRNA on the panel, refer to the plate layout file.
The Cancer Focus miRNA PCR Panel is available in:
Both formats are ready-to-use with 10 µl reactions per well.
The panel contains assays for the following:
The Cancer Focus miRNA PCR Panels are compatible with most real-time PCR instruments (see table below).
Exosome Focus miRNA PCR PanelsKey benefits of the Exosome Focus miRNA PCR Panels include:
The Exosome Focus miRNA PCR Panels are offered as pre-defined Custom miRNA PCR Panels. This includes pre-defined lists of assays for miRNAs expressed in either exosomes from urine or exosomes from cerebrospinal fluid (CSF). Both groups of assays are designed to fit into a single 96-well PCR plate or as 4 replicates in a 384-well PCR plate.
The Urine Exosome Focus miRNA PCR Panel contains:
The CSF Exosome Focus miRNA PCR Panel contains:
The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green Master Mix and aliquot into the 96- or 384-well PCR plate containing the pre-aliquoted PCR Panel. The plate is now ready to run in your real-time PCR instrument.
miRNA quantification in serum, plasma, exosomes, urine and other liquid biopsies; biomarker discovery
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