QIAshredder

简单快速匀浆细胞和组织的裂解液

  • 代替针筒抽吸的匀浆步骤
  • 可以减少样品的消耗
  • 防止样本间的交叉污染
  • 可以去除不溶性沉淀碎片和降低粘稠度
QIAshredder的离心柱中含有独特的生物聚合物剪切体系。将细胞或组织裂解物上样到置于收集管中的QIAshredder并进行离心,从而收集到匀浆的裂解物。一般来说,所得RNA的产量与质量与转子-定子匀浆方法相当(参见"Comparison of RNA yields")。
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QIAshredder (250)
250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79656
€ 508,00
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QIAshredder (50)
50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79654
€ 113,00
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The QIAshredder is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Kit Handbooks
1
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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Images
Comparison of RNA yields with different homogenization methods

Comparison of RNA yields.

Northern blot of total RNA isolated from 5 x 106 HeLa cells using the RNeasy Mini procedure with the indicated homogenization methods. RNA was eluted with 2 x 40 µl water, and 10 µl was loaded per lane.