QIAshredder

For simple and rapid homogenization of cell and tissue lysates
  • Replaces syringe-and-needle homogenization
  • Reduces loss of sample material
  • Eliminates cross-contamination between samples
  • Filters out insoluble debris and reduces viscosity
The QIAshredder homogenizer consists of a unique biopolymer-shredding system in a microcentrifuge spin-column format. Cell or tissue lysate is loaded onto the QIAshredder homogenizer placed in a collection tube and centrifuged. The homogenized lysate is then collected. In general, similar yields and quality of RNA are obtained as with rotor–stator homogenization (see figure "Comparison of RNA yields").
製品名 Cat. no. List price:
QIAshredder (250)
250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79656
¥55,000
QIAshredder (50)
50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79654
¥12,000

QIAshredder  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


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キットハンドブック
1
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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画像
Comparison of RNA yields with different homogenization methods

RNA収量の比較

5 x 106個のHeLa細胞からRNeasy Miniを用いて上記のホモジナイゼーション法により精製したRNAのノーザンブロット。RNA は40 µlの水で2回溶出し、レーンあたり10 µlを電気泳動した。