Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666

Yes. If Library QC suggests that the library is of good quality and simply low in concentration, it can still be used for sequencing. It is possible to either sequence the library at 2nM or otherwise at the maximum amount available for that library (either individually or in multiplex with other samples). It is recommended to  keep all libraries being multiplexed at comparable concentrations