The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A]1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B]1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.
GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
QIAvac 24 plus.
Manifold with luer connectors.
Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit is shown.