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EZ1 DNA Cards

For easy setup of protocols using EZ1 DNA Blood Kits and the EZ1 DNA Tissue Kit on the BioRobot EZ1

Features

  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results
  • Optimized protocols help to ensure reliable purification
  • Automated purification of genomic DNA

Useful Resources

EZ1&2 DNA Tissue Handbook EZ1&2 DNA Blood Handbook EZ1&2 DNA Blood Kit Quick-Start Protocol
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EZ1 DNA Blood Card

Cat. No. / ID: 9015585

Pre-programmed card for BioRobot EZ1 DNA Blood 200 µl and 350 µl Protocols
 DKK 6,670.00
Sample type
Blood
Dried Blood
Tissue
Buccal Swab
Buffy Coat
Paraffin Section
Bacteria
EZ1 Cards are used in combination with EZ1 Kits on the BioRobot EZ1. Please refer to the specific EZ1 Kit handbook and the BioRobot EZ1 User Manual for information about product use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

EZ1 DNA Cards are preprogrammed with protocols for purification of DNA from blood and blood-derived samples, dried blood, tissues, buffy coat, paraffin-embedded tissues, buccal swabs, human samples, swabs, biopsies and bacterial cultures.

Performance

The yield and quality of the purified DNA are suited for use in many applications (see figures  "Representative yields of high-purity DNA from buffy coat samples",  "Consistent PCR results",  "Reproducible performance in sensitive PCR analysis" and  "Sensitive PCR using DNA purified from preserved blood", and also table "DNA yields obtained from bacterial suspension cultures").

DNA yields obtained from bacterial suspension cultures

Sample type Gram Optical density
(OD) value		 Starting volume DNA yield (µg)
Escherichia coli – 0.7 200 µl 6.6 ± 0.4
Pseudomonas spp. – 0.7 200 µl 9.0 ± 0.5
Bacillus subtilis + 0.4 1000 µl* 5.7 ± 0.2
Staphylococcus spp. + 0.2 1000 µl* 5.7 ± 0.2
 
* Bacteria cultures were pelleted and resuspended in PBS.
See figures
Representative yields of high-purity DNA from buffy coat samples.Representative yields of high-purity DNA from buffy coat samples.Consistent PCR results.Consistent PCR results.Reproducible performance in sensitive PCR analysis.Reproducible performance in sensitive PCR analysis.Sensitive PCR using DNA purified from preserved blood.Sensitive PCR using DNA purified from preserved blood.

Principle

EZ1 Cards specify a choice of purification protocols without any manual data entry. Additional EZ1 Cards may be purchased at any time to increase the application range of the BioRobot EZ1.

Procedure

Simply insert the EZ1 Card with the desired protocol into the EZ1 Card slot and start the instrument.

Applications

The high-quality DNA obtained using the BioRobot EZ1 and EZ1 DNA Cards with EZ1 DNA Blood Kits and the EZ1 DNA Tissue Kit is suitable for use in a wide range of applications, such as:

  • Genotyping analysis, including STR, SNP, VNTR, RAPD, NASBA and AFLP technologies
  • Population genetics and pharmacogenomic studies
  • Oncology and infectious disease research
  • Purification of DNA from archived samples
  • PCR and real-time RT-PCR
  • Molecular testing research
  • Sequencing
  • Bacterial detection research
  • Epidemiology and infection control
  • Quantification and resistance profiling by PCR
  • Forensic research

Features Specifications
Applications PCR, real-time RT-PCR, genotyping
For automated processing BioRobot EZ1
Processing Automated

Supporting data and figures

Representative yields of high-purity DNA from buffy coat samples.

Each data point represents the average yield from 3 replicates.
Representative yields of high-purity DNA from buffy coat samples.
Representative yields of high-purity DNA from buffy coat samples.
Consistent PCR results.
Reproducible performance in sensitive PCR analysis.
Sensitive PCR using DNA purified from preserved blood.

Resources

Quick-Start Protocols (2)
EZ1&2 DNA Blood Kit Quick-Start Protocol
PDF (903KB)
EZ1&2 DNA Tissue Kit Quick-Start Protocol
PDF (881KB)
Application Notes (8)
Automated DNA preps from soft tissue biopsies and mouse tails using the BioRobot EZ1 System - (EN)
PDF (484KB)
Automated purification of DNA from bones of a Bronze Age family using the BioRobot EZ1 workstation
PDF (1MB)
Automated purification of DNA from buffy coat samples using the BioRobot EZ1 System - (EN)
PDF (480KB)
(EN) - Comparison of enzymes to lyse streptococcal bacteria priorto automated DNA purification on the BioRobot M48
PDF (168KB)
(EN) - Efficient purification of high-quality DNA from blood samples with a range of white cell counts using the BioRobot EZ1 System
PDF (477KB)
High-performance DNA from forensic samples using the EZ1 DNA Tissue Kit together with the EZ1 DNA Forensic Card protocols - (EN)
PDF (411KB)
(EN) - Reproducible purification of DNA from blood using the BioRobot EZ1 System
PDF (500KB)
(EN) - Routine use of the BioRobot EZ1 in detection of veterinary and zoonotic pathogens
PDF (448KB)
Supplementary Protocols (1)
Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA® assays, using EZ1® DNA Blood Kits - (EN)
PDF (76KB)
Kit Handbooks (2)
EZ1&2 DNA Tissue Handbook
PDF (672KB)
EZ1&2 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments
EZ1&2 DNA Blood Handbook
PDF (568KB)
EZ1&2 DNA Blood 200 μl Kit
EZ1&2 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments
User-Developed Protocols (1)
Purification of DNA from fungi using the EZ1 DNA Tissue Kit - (EN)
PDF (36KB)

FAQ

Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
Yes, please follow the Supplementary Protocol 'Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA® assays, using EZ1® DNA Blood Kits’.
FAQ ID -1169
Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?

Yes, please follow the Supplementary Protocol 'Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit' (MA15).

FAQ ID -990
How should I prepare buffy coat samples for use on the BioRobot EZ1?

Making consistent buffy samples takes practice. Serum and erythrocytes are easily isolated alongside the 10x concentrated leukocyte fraction. Our Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02) contains instructions and recommendations for preparation of buffy coat for DNA isolation on the BioRobot EZ1.

FAQ ID -1249
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?

Yes, please check the EZ1 DNA Tissue Handbook for instructions on pretreatment and extraction protocol.

 

FAQ ID -1260
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).
FAQ ID -992
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246

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