Products

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Autopure Qubes D (192)

Cat. No. / ID: 949022

192 Autopure Qubes D and Caps
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Autopure Glycogen Solution (5 ml)

Cat. No. / ID: 949002

5 ml Autopure Glycogen Solution
This product will be discontinued as of March 31, 2023 or until stocks last. We recommend 158186.
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Autopure Cell Lysis Solution (3800 ml)

Cat. No. / ID: 949006

3800 ml Autopure Cell Lysis Solution
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Autopure Precipitation Solution (3800 ml)

Cat. No. / ID: 949008

3800 ml Autopure Precipitation Solution (for proteins)
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Autopure 100% Isopropanol (3800 ml)

Cat. No. / ID: 949016

3800 ml Autopure 100% Isopropanol
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Autopure 70% Ethanol (3800 ml)

Cat. No. / ID: 949018

3800 ml Autopure 70% Ethanol
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Autopure DNA Hydration Solution (3800 ml)

Cat. No. / ID: 949010

3800 ml Autopure DNA Hydration Solution
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Autopure Qubes E (192)

Cat. No. / ID: 949020

192 Autopure Qubes E and Caps
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Autopure RBC Lysis Solution (9500 ml)

Cat. No. / ID: 949004

9500 ml Autopure RBC Lysis Solution
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Autopure RNase A Solution (19 ml)

Cat. No. / ID: 949014

19 ml Autopure RNase A Solution
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Autopure Waste Container

Cat. No. / ID: 9017686

Waste container for the Autopure LS

Product Details

Autopure reagents and accessories enable automated purification of genomic DNA from large-volume samples on the Autopure LS. Purified DNA is highly stable and well-suited for archiving.

Resources

Supplementary Protocols (14)
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (1)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, body fluids, cultured cells, and tissue

Publications

Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.
Hoyo C; Murtha AP; Schildkraut JM; Jirtle RL; Demark-Wahnefried W; Forman MR; Iversen ES; Kurtzberg J; Overcash F; Huang Z; Murphy SK;
Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975
Genetic variation in the growth hormone synthesis pathway in relation to circulating insulin-like growth factor-I, insulin-like growth factor binding protein-3, and breast cancer risk: results from the European prospective investigation into cancer and nutrition study.
Canzian F; McKay JD; Cleveland RJ; Dossus L; Biessy C; Boillot C; Rinaldi S; Llewellyn M; Chajès V; Clavel-Chapelon F; Téhard B; Chang-Claude J; Linseisen J; Lahmann PH; Pischon T; Trichopoulos D; Trichopoulou A; Zilis D; Palli D; Tumino R; Vineis P; Berrino F; Bueno-de-Mesquita HB; van Gils CH; Peeters PH; Pera G; Barricarte A; Chirlaque MD; Quirós JR; Larrañaga N; Martínez-García C; Allen NE; Key TJ; Bingham SA; Khaw KT; Slimani N; Norat T; Riboli E; Kaaks R;
Cancer Epidemiol Biomarkers Prev; 2005; 14 (10):2316-25 2005 Oct PMID:16214911

FAQ

What is the composition of RBC Lysis Solution?
The exact composition of RBC Lysis Solution is confidential. RBC Lysis Solution is a component of Gentra Puregene Blood Kits. RBC Lysis Solution selectively lyses human red blood cells leaving white blood cells intact. You can order RBC Lysis Solutin separately.
FAQ ID -2808
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

The composition of DNA Hydration Solution is as follows:

  • 10 mM Tris
  • 1 mM EDTA
  • pH 7–8

DNA Hydration Solution is included in QIAGEN’s Gentra Puregene Kits for DNA purification and can also be bought separately from QIAGEN.

FAQ ID -2813
What is the composition of Cell Suspension Solution?
The detailed composition of Cell Suspension Solution is confidential. Cell Suspension Solution is a component of the Gentra Puregene Yeast/Bacteria Kit. Essentially, it is an isotonic solution that prevents spheroblasts from bursting. Cell Suspension Solution can be ordered separately.
FAQ ID -2811
What is the composition of Protein Precipitation Solution?
The exact composition of Protein Precipitation Solution is confidential. Protein Precipitation Solution is a component of Gentra Puregene Kits for DNA purification. Essentially, this is a high-salt buffer that lowers the solubility of proteins. Protein Precipitation Solution can be ordered separately.
FAQ ID -2810
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
The exact composition of Glycogen Solution is confidential. The Glycogen concentration is 20mg/mL. Glycogen Solution is a component of Gentra Puregene Kits for DNA purification. During isopropanol precipitation, Glycogen Solution acts as a nucleic acid carrier and helps to efficiently precipitate small amounts of DNA. In addition, it facilitates visualization of the DNA pellet. Glycogen Solution can be purchased separately.

 

Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.

 

 

FAQ ID -2812
How can samples be shipped and stored prior to extraction on the Autopure LS?
Precautions:
Mix blood well after collection into Vacutainer tube to prevent small blood clots from forming.
• Place blood samples on ice or at 4ºC until addition of RBC Lysis Solution.
• If storing samples, place at either 4ºC or -80ºC within 24hours.
• Do not ship samples at ambient temperature. Always ship fresh blood samples overnight on ice. If samples are frozen, ship as quickly as possible on dry ice to prevent the blood from thawing during transport.
• Do not store at -20ºC. Storing blood at -20ºC can result in lower yields and quality of DNA when compared to -80ºC stored blood.
• Do not freeze blood in glass Vacutainer tubes. Blood may be frozen a 15 ml or 50 ml centrifuge tube.


Sample Storage: Short term (up to 5 days) • Store blood at 4ºC for up to 5 days.
• Store blood for no longer than 24 hours at room temperature. Long-term (up to 2 years)
• For long-term storage, store blood at -70ºC or -80ºC. It is helpful if the blood is stored in plastic tubes in aliquots that will be used for subsequent purification. Blood frozen at -80ºC may be stored for up to 2 years. *Note: DNA yields are reduced by approximately 20% when blood is frozen at -80ºC.
• Do not store blood at –20ºC. Storing blood at this temperature will compromise the samples resulting in reduced yields and quality of the DNA.
FAQ ID - 3522
How can samples be shipped and stored prior to extraction on the Autopure LS?

For shipping and storage of samples prior to extraction on the Autopure LS, we recommend the following:

Precautions:

Mix blood well after collection into Vacutainer tube to prevent small blood clots from forming.

• Place blood samples on ice or at 4ºC until addition of RBC Lysis Solution.

• If storing samples, place at either 4ºC or -80ºC within 24hours.

• Do not ship samples at ambient temperature. Always ship fresh blood samples overnight on ice. If samples are frozen, ship as quickly as possible on dry ice to prevent the blood from thawing during transport.

• Do not store at -20ºC. Storing blood at -20ºC can result in lower yields and quality of DNA when compared to -80ºC stored blood.

• Do not freeze blood in glass Vacutainer tubes. Blood may be frozen a 15 ml or 50 ml centrifuge tube.

Sample Storage:

Short term (up to 5 days) • Store blood at 4ºC for up to 5 days.

• Store blood for no longer than 24 hours at room temperature. Long-term (up to 2 years) • For long-term storage, store blood at -70ºC or -80ºC. It is helpful if the blood is stored in plastic tubes in aliquots that will be used for subsequent purification. Blood frozen at -80ºC may be stored for up to 2 years. *Note: DNA yields are reduced by approximately 20% when blood is frozen at -80ºC.

• Do not store blood at –20ºC. Storing blood at this temperature will compromise the samples resulting in reduced yields and quality of the DNA.

FAQ ID - 3528
What is the composition of Cell Lysis Solution?
The exact composition of Cell Lysis Solution is confidential. Cell Lysis Solution is a component of Gentra Puregene Kits for DNA purification. It functions by enabling cell lysis. Cell Lysis Solution can be purchased separately.
FAQ ID -2809
What do you recommend for the cleanup of genomic DNA (gDNA)?

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

 

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

 

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618