EZ1 DNA Investigator Cards

EZ1 DNA Investigator Cards are preprogrammed with protocols for purification of DNA from forensic and human-identity samples using the EZ1 DNA Investigator Kit on the EZ1 Advanced, EZ1 Advanced XL or BioRobot EZ1.

EZ1 DNA Kits when used in combination with EZ1 DNA Investigator Cards and EZ1 instruments provide rapid DNA purification from forensic samples, e.g., trace samples, cigarette butts and hair.

Easier handling of solid materials with “tip dance” protocol

In addition, EZ1 DNA Investigator Cards provide an optional, fully automated “tip dance” protocol on EZ1 instruments, where the filter-tip moves back-and-forth relative to the worktable platform while pipetting. This enables processing of solid materials, such as swabs, fabrics, blood discs or cigarette butts, directly in the sample tube. There is no need for prior centrifugation to remove solid materials that could clog the tip (see flowchart  "EZ1 DNA Investigator trace and “tip dance” protocols"). The “tip dance” protocol simplifies handling and, in some cases, increases DNA yield with more efficient extraction of the sample (see figure  "Easy and efficient processing of solid materials with the “tip dance” protocol").

Optimized protocol for larger sample volumes

A large-volume protocol enables fully automated processing of starting volumes up to 500 µl on EZ1 instruments. This not only allows efficient DNA purification from dilute samples with low concentrations of DNA, such as diffuse stains, but also enables purification from samples that require larger volumes for thorough lysis. The ability to process larger sample volumes – with the same elution volume as the standard trace protocol – enables higher yields of more concentrated DNA for greater sensitivity in downstream applications (see  "Higher yields of concentrated DNA with the large-volume protocol").

EZ1 DNA Investigator Cards specify a choice of purification protocols without any manual data entry. Additional EZ1 Cards may be purchased at any time to increase the application range of EZ1 instruments.

Simply insert the EZ1 Card with the desired protocol into the card slot and start the EZ1 instrument.

The high-quality DNA obtained using EZ1 instruments with EZ1 DNA Investigator Cards is suited for use in many applications, including:

• Genetic identity testing and forensics
• Purification of DNA from trace samples, such as casework or crime-scene samples
• Routine analysis of reference samples
• Multiplex PCR and real-time PCR
• Genotyping analysis, including SNP, STR or NGS technologies