EZ1 DNA Kits when used in combination with EZ1 DNA Investigator Cards and EZ1 instruments provide rapid DNA purification from forensic samples, e.g., trace samples, cigarette butts and hair.
Easier handling of solid materials with “tip dance” protocol
In addition, EZ1 DNA Investigator Cards provide an optional, fully automated “tip dance” protocol on EZ1 instruments, where the filter-tip moves back-and-forth relative to the worktable platform while pipetting. This enables processing of solid materials, such as swabs, fabrics, blood discs or cigarette butts, directly in the sample tube. There is no need for prior centrifugation to remove solid materials that could clog the tip (see flowchart "EZ1 DNA Investigator trace and “tip dance” protocols"). The “tip dance” protocol simplifies handling and, in some cases, increases DNA yield with more efficient extraction of the sample (see figure "Easy and efficient processing of solid materials with the “tip dance” protocol").
Optimized protocol for larger sample volumes
A large-volume protocol enables fully automated processing of starting volumes up to 500 µl on EZ1 instruments. This not only allows efficient DNA purification from dilute samples with low concentrations of DNA, such as diffuse stains, but also enables purification from samples that require larger volumes for thorough lysis. The ability to process larger sample volumes – with the same elution volume as the standard trace protocol – enables higher yields of more concentrated DNA for greater sensitivity in downstream applications (see "Higher yields of concentrated DNA with the large-volume protocol").