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ipsogen BCR-ABL1 Mbcr IS-MMR Kits

For the quantification of BCR-ABL1 Mbcr b3a2 & b2a2 fusion gene transcripts and results alignment on the international scale
  • Results on the international scale (IS) and MMR reporting
  • One IS-MMR calibrator per run to prevent drifting
  • High reliability using EAC technology and single plasmid standards
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection and quantitation of BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression.
Cat No./ID: 670713
ipsogen BCR-ABL1 Mbcr IS-MMR Kit (24)
For 24 reactions: High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene
Cat No./ID: 670813
ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit (24)
For 24 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT, High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene, qPCR Master mix, ROX II fluorescent dye
0
Single plasmid for BCR-ABL gene transcript.
1
ABL control gene transcript.
2
Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator.
Mbcr plasmid standards (SP1-SP6), 101, 102, 103, 105, and 106 copies/5 μl.
3
Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator.
ABL plasmid standards (SP3–SP6), 103, 104, 105, and 106 copies/5 μl.
Performance
To ensure highest sensitivity, ipsogen BCR-ABL1 Mbcr IS-MMR Kits have been optimized to detect low levels of BCR-ABL p210 b2a2 or b3a2 transcripts (see figures Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator and Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator). The sensitivity has a limit of detection (LOD) of 0.0069.

Principle
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are ready-to-use kits for the detection of BCR-ABL p210 b2a2 or b3a2 transcripts using real-time PCR. The kits are based on the amplification and detection of specific BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. The kits provide high levels of specificity, sensitivity, and reproducibility. Each ipsogen BCR-ABL1 Mbcr IS-MMR Kit provides 4 standard dilutions for ABL and 5 standard dilutions for Mbcr. Use of a single plasmid for BCR-ABL1 and ABL enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations (see figures Single plasmid for BCR-ABL gene transcript and ABL control gene transcript). The kits also include an IS-MMR calibrator allowing conversion of results to the international scale for accurate measurement of molecular response and major molecular response (MMR) status reporting. A high positive control RNA is also included to monitor the reverse transcription and amplification steps of ABL and BCR-ABL Mbcr during transcript quantification. The ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit includes reagents for reverse transcription-PCR and real-time PCR.


Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using ipsogen BCR-ABL1 Mbcr IS-MMR Kits allows detection and quantification of BCR-ABL p210 b2a2 or b3a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications
The ipsogen BCR-ABL1 Mbcr IS-MMR Kits enable sensitive and reliable detection and quantification of BCR-ABL p210 b2a2 or b3a2 transcripts, conversion of results to the international scale, and MMR reporting, for research use only.
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