QIAGEN Protease and Proteinase K

For protease digestion during DNA and RNA preparation

S_1280_3_LS_OEM_QIAGEN_Protease_30_AU
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QIAGEN Protease (30 AU)

Cat. No. / ID:  19157

4 x 7.5 Anson units per vial (lyophilized)
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DKK 2,780.00
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EnzymeSolution
QIAGEN Protease
QIAGEN Proteinase K
QIAGEN Protease Solvent
Contains
30 (4 x 7.5) AU
7.5 AU
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM

Features

  • For protease digestion in DNA and RNA isolation procedures
  • Fully compatible with selected QIAGEN protocols
  • Both enzymes quality-guaranteed by QIAGEN

Product Details

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

Performance

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures. Both enzymes are quality-guaranteed by QIAGEN. 

QIAGEN Protease is particularly stable and active at high pH. In Tris-containing buffers of alkaline pH (7.5 - 10.5) QIAGEN Protease displays 30 to 40% higher specific activity than Proteinase K. 
In the presence of strong denaturants, such as urea (0.75 - 3 M urea) or guanidine HCl (0.5 - 3.0 GuHCl), the specific activity is comparable to that of Proteinase K, provided the EDTA concentration is less than 8 mM. 
QIAGEN Protease shows increased activity in the presence of urea and guanidine HCl. Similar stimulation is obtained upon the addition of up to 1% SDS. Enzymatic activity also increases as a function of 
temperature (30-55°C). For more detailed information, refer to the QIAGEN Protease Product Sheet in the Resources section.

The enzyme can be easily inactivated by incubation at 70°C for 15 minutes.
QIAGEN Protease is not inhibited by up to 100 mM EDTA in Tris·Cl buffers. However, in the presence of greater than 1% SDS or other strong denaturants, the EDTA concentration must be less than 8 mM.

Principle

Technical specifications

  QIAGEN Protease QIAGEN Proteinase K
Format Lyophilized powder Ready-to-use solution
Amount 7.5 AU or 4 x 7.5 AU 2 ml or 10 ml (20 mg/ml)
Activity 45 mAU/mg protein >600 mAU/ml
Unit definition One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute

QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:

  • QIAamp DNA Mini Kit
  • QIAamp Fast DNA Stool Mini Kit
  • QIAamp 96 DNA Swab BioRobot Kit
  • DNeasy Blood & Tissue Kit
  • DNeasy 96 Blood & Tissue Kit
  • QIAamp DNA Micro Kit
  • QIAamp MinElute Media Kit
  • QIAamp UltraSens Virus Kit
  • QIAamp Media MDx Kit

QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:

  • QIAamp DNA Blood Mini, Midi and Maxi Kits
  • QIAamp 96 DNA Blood Kit
  • Blood & Cell Culture DNA Kits
  • QIAamp DNA Blood BioRobot 9604 Kit
  • QIAamp Virus BioRobot 9604 Kit
  • QIAamp DNA Blood BioRobot MDx Kit
  • QIAamp DSP Virus Kit
  • QIAamp DSP DNA Blood Mini Kit

Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.

Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.

Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.

Procedure

Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.

Applications

QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

FAQ ID - 3447
How can QIAGEN Protease and Proteinase K be inactivated?

QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.

To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.

FAQ ID -315
3192 - Is QIAGEN Protease compatible with Buffer ATL?
No, QIAGEN Protease is not compatible with Buffer ATL.