ハンディータイプのロースループットサンプルの破砕
- 様々なタイプのサンプルを迅速かつ効果的に破砕
- ディスポーザブルプローブでクロスコンタミを回避
- ディスポーザブルプローブで時間を節約
- プローブは透明なのでサンプル破砕を目視で確認可能
- QIAGEN サンプル精製キットの前処理として対応確認済み
TissueRuptor はローターステーター型のハンディータイプのホモジナイザーで、幅広いアプリケーション用にヒト、動物、植物サンプルを迅速かつ効果的に破砕します。TissueRuptorの透明なディスポーザブルプローブはクロスコンタミのリスクを最小限に抑え、またサンプルの破砕工程を目で確認することができます。ディスポーザブルプローブを使用することにより、各サンプルのホモジナイゼーション後にプローブを洗浄する必要がないため、時間も節約できます。
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TissueRuptor II (230 V, 50/60 Hz, EU/CH)
Handheld rotor–stator homogenizer, 230 V, 50/60 Hz (for Europe [excluding UK and Ireland]), 5 TissueRuptor Disposable Probes
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9002756
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TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
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990890
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The TissueRuptor II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Reproducible purification of high-quality genomic DNA.|Efficient disruption and homogenization of animal tissues.|Successful purification of phosphoproteins.|High performance results in PCR.|Pure RNA with high RIN values.|
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.|The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.|Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.|[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.|Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.|
Principle
ジェノタイピング、遺伝子発現解析、プロテオミクスにおける実験ではDNA、RNA、タンパク質を確実に高収量で得るために生体サンプルを効果的に破砕する必要があります。TissueRuptorシステムは、生体分子を完全に遊離するため、迅速かつ完璧にサンプルを破砕し、さらにQIAGEN 製品(表参照)を用いて容易に精製を行なえるよう、同時にサンプルをホモジナイズします。
QIAGEN はサンプル採取からDNA、RNA、タンパク質の精製および解析までのトータルソリューションを提供しており、TissueRuptorはその破砕工程を構成しています。サンプル破砕と生体分子の精製が一緒になった至適化済みのプロトコールにより、迅速かつ効率的なワークフローを実現します。さらに、ロースループットからハイスループット数のサンプルから生体分子の精製と解析を行なう各種自動化用製品も取り揃えています。
QIAGEN はまたRNA 安定化のための RNAlater RNA Stabilization Reagent およびDNA、RNA、タンパク質を安定化するための Allprotect Tissue Reagentもお届けしています。これらの試薬中に採取した組織はTissueRuptor で容易に破砕することが可能です。
| RNA |
溶解が容易な組織 |
RNeasy Kits
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| RNeasy Plus Kits |
| RNeasy Protect Kits |
| RNA |
繊維性組織 |
RNeasy Fibrous Tissue Kits |
| RNA |
全タイプの組織 |
RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits |
| RNA |
植物組織 |
RNeasy Plant Mini Kit |
| RNA |
酵母 |
RNeasy Kits |
| RNA |
バクテリア |
RNeasy Protect Bacteria Kits |
| microRNA |
全タイプの組織 |
miRNeasy Kits |
| DNA |
ヒト組織 |
QIAamp DNA Kits |
| DNA |
動物組織 |
DNeasy Blood & Tissue Kits |
| タンパク質 |
組織 |
Qproteome Mammalian Protein Prep Kit |
| リン酸化タンパク質 |
組織 |
PhosphoProtein Purification Kit |
| 糖タンパク質 |
組織 |
Qproteome Glycoprotein Kits |
| DNA およびRNA |
組織 |
AllPrep DNA/RNA Kits |
| DNA、RNA、タンパク質 |
組織 |
AllPrep DNA/RNA/Protein Mini Kit |
Procedure
TissueRuptor はハンディータイプのホモジナイザーで、TissueRuptor Disposable Probe を装着して使用します。プローブの刃の高速回転により生じる乱流と機械的剪断の組み合わせがサンプルを破砕、ホモジナイズします。ヒト、動物、植物組織などのサンプルを処理します(植物組織の破砕は難しいので TissueLyser II を推奨)。スタートサンプルから核酸あるいはタンパク質を遊離するためには、通常、最高速度でわずか30 秒の破砕操作で十分です。
TissueRuptor Disposable Probe はプローブを再利用するための洗浄が不要なので、時間を節約しクロスコンタミを回避します。さらに TissueRuptor Disposable Probe は透明なため、サンプルの破砕工程を目で確認することができます。
Applications
TissueRuptorは、凝固血液を含むヒト、植物、動物サンプルを迅速かつ効果的に破砕します。破砕後、RNA、DNA、トータル核酸、タンパク質はQIAGENキットを用いて精製可能です。
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Download Safety Data Sheets for QIAGEN product components.
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View
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For low-throughput disruption of biological samples using disposable probes
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Show details
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Images
Reproducible purification of high-quality genomic DNA.
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.
Efficient disruption and homogenization of animal tissues.
The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.
Successful purification of phosphoproteins.
Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.
High performance results in PCR.
[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.
Pure RNA with high RIN values.
Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.
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