MinElute 96 UF PCR Purification Kit

For high-throughput, ultrafiltration purification of up to 15 μg PCR products (≥100 bp)

  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries

The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products ≥100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 μl) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).
The product must be used in conjunction with QIAvac Multiwell.

产品 货号 目录价:
MinElute 96 UF PCR Purification Kit (4)
4 MinElute 96 UF PCR Purification Plates
28051
询价
MinElute 96 UF PCR Purification Kit (24)
24 MinElute 96 UF PCR Purification Plates
28053
询价

MinElute 96 UF PCR Purification Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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MinElute 96 UF PCR Purification实验流程。|DNA洗脱液浓度高。|
使用手动流程,可在18分钟内制备96个样本。|使用MinElute 96 UF PCR Purification Kit对浓缩DNA进行高效纯化。使用MinElute 96 UF PCR Purification Plates(QIAGEN)或其他供应商(Supplier MIIISupplier M)提供的超滤试剂盒纯化DNA片段(100 bp和500 bp)。利用琼脂糖凝胶电泳分析少量(5 μl)洗脱液。M:分子量标准。|
性能
The MinElute 96 UF PCR Purification Kit provides high-throughput PCR purification in 96-well plates. Fully automated processing is provided for 1–8 x 96 samples on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System, or for 1 x 96 samples on the QIAvac Multiwell Manifold (cat. no. 9014579, requires suitable vacuum manifold, e.g., QIAvac Multiwell manifold, plate shaker recommended). The procedure purifies DNA ≥100 bp in size with a recovery of 80–95%. Purified PCR products are eluted directly from the surface of the membrane in small volumes (as little as 20 µl in the manual procedure or 30 µl on BioRobot workstations), providing high-concentration eluates (see figure "Highly concentrated DNA eluates") that are suitable for direct use in a variety of downstream applications.  It is possible to process PCR sample volumes up to 150 µl using either a manual or fully automated procedure.
原理

The QIAGEN MinElute 96 UF PCR Purification Kit combines the convenience of multiwell technology and the separation properties of an ultrafiltration membrane with a unique design. The plates enable the fast and efficient recovery of double-stranded DNA fragments ≥100 bp and the removal of primers (<20mers), unincorporated nucleotides, and salts.

操作流程
MinElute 96 UF PCR Purification Kits provide ultrafiltration technology using vacuum-driven purification on QIAvac manifolds (see flowchart "MinElute 96 UF PCR Purification procedure"). PCR products are loaded into the wells of the ultrafiltration plates, and a vacuum is applied. While small molecules such as primers, salts, and unincorporated nucleotides pass through the membrane, PCR products ≥100 bp are retained. Water, DMSO, or buffers such as SSC can be used for elution. The MinElute 96 UF PCR Purification procedure can also be automated on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System.
应用

DNA fragments purified with the MinElute 96 UF system are ready for direct use in many applications, including:

Sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling

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For efficient, high-throughput purification of PCR products
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MinElute 96 UF PCR Purification Procedure
MinElute 96 UF PCR Purification实验流程。
使用手动流程,可在18分钟内制备96个样本。
Highly Concentrated DNA
DNA洗脱液浓度高。
使用MinElute 96 UF PCR Purification Kit对浓缩DNA进行高效纯化。使用MinElute 96 UF PCR Purification Plates(QIAGEN)或其他供应商(Supplier MIIISupplier M)提供的超滤试剂盒纯化DNA片段(100 bp和500 bp)。利用琼脂糖凝胶电泳分析少量(5 μl)洗脱液。M:分子量标准。