Factor Xa Protease
For endoproteolytic removal of short 6xHis affinity tags from 6xHis-tagged recombinant proteins
Factor Xa Protease is a site-specific endoprotease that preferentially cleaves the C-terminal peptide bond of the recognition sequence Ile-Glu-Gly-Arg. Therefore, Factor Xa Protease efficiently cleaves 6xHis-tagged proteins expressed by the pQE-30 Xa vector as the 6xHis tag precedes the recognition sequence, giving protein free of vector-encoded amino acids. After cleavage of the purified protein, Factor Xa Protease is removed in a batch procedure using Xa Removal Resin.
The Factor Xa Protease is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Sample to Insight
Location:
Deutschland
Language:
Deutsch
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Factor Xa Protease
For endoproteolytic removal of short 6xHis affinity tags from 6xHis-tagged recombinant proteins
- Efficient 6xHis tag cleavage: >90%
- Protein free of vector-encoded amino acids from pQE-30 Xa vector
Factor Xa Protease is a site-specific endoprotease that preferentially cleaves the C-terminal peptide bond of the recognition sequence Ile-Glu-Gly-Arg. Therefore, Factor Xa Protease efficiently cleaves 6xHis-tagged proteins expressed by the pQE-30 Xa vector as the 6xHis tag precedes the recognition sequence, giving protein free of vector-encoded amino acids. After cleavage of the purified protein, Factor Xa Protease is removed in a batch procedure using Xa Removal Resin.
The Factor Xa Protease is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features |
Specifications |
| Applications | Protein crystallography, structure determination studies using NMR |
| Efficiency of Tag removal | >90% |
| Protease recognition site | Factor Xa Protease recognition site |
| Special feature | Protein free of vector-encoded amino acids |
| Tag removal | Endoproteolytic |